Primer

Part:BBa_K1456008

Designed by: safa tapan   Group: iGEM14_ATOMS-Turkiye   (2014-08-13)


Superoxide Dismutase enzyme reverse primer with restriction site, two stop codon and 6X His-Tag

we use this primer to prepare our Superoxide dismutase-1SOD-1)enzyme from cDNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 14
    Illegal PstI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 14
    Illegal PstI site found at 2
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 14
    Illegal PstI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 14
    Illegal PstI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aSuperoxide Dismutase enzyme reverse primer with restriction site, two stop codon and 6X His-Tag